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| Roch, Philippe. |
| Durant de nombreuses années, faire de la biologie a consisté en la description des processus qui gouvernent le monde vivant. Le développement de la biochimie et surtout l'explosion récente de la biologie moléculaire en ont fait une science d'intervention permettant de modifier les organismes vivants. |
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| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/publication-7480.pdf |
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| Mitta, Guillaume; Vandenbulcke, Franck; Noel, Thierry; Romestand, Bernard; Beauvillain, Jean Claude; Salzet, Michel; Roch, Philippe. |
| In previous papers, we characterised 3 types of 4-kDa, cysteine-rich, cationic antimicrobial peptides: MGDs (for Mytilus galloprovincialis defensins), mytilins and myticins, which are abundant in the mussel hemocytes. In the present work, me revealed a differential distribution of the cells expressing the different genes. In addition, using confocal and electron microscopy, we confirmed that defensins and mytilins were partially located in different sub-types of circulating hemocytes although the peptides can be located in the same cell, and even in the same granule. We also demonstrated that mytilins exert their microbicidal effect within the cells through the process of phagosome-mytilin granule fusion leading to the co-location of ingested bacteria and... |
| Tipo: Text |
Palavras-chave: Innate immunity; Hemocyte; Mussel; Myticin; Mytilin; Defensin; Antimicrobial. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-413.pdf |
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| Li, Hui; Toubiana, Mylene; Monfort, Patrick; Roch, Philippe. |
| Several bivalves, including mussels, suffered from mortalities particularly in summer. To look for the possible effect of environmental parameters on immune capacities, Mytilus galloprovincialis were collected monthly from August 2005 to July 2008 from the Palavas Laguna, French Mediterranean coast. Q-PCR was used to quantify the expression of three antimicrobial peptide genes (defensin, mytilin B and myticin B), in addition to lysozyme and HSP70. House keeping gene was 28S rRNA. Defensin, myticin B and lysozyme appeared more expressed in spring-summer than in winter. In contrast, HSP70 expression was higher in winter. Statistical studies using principal component analysis (PCA) and multiple regression models revealed positive influence of temperature on... |
| Tipo: Text |
Palavras-chave: Mollusks; Mytilus; Innate immunity; Seasonal variations; Immune survey; Q PCR; HSP70; Lysozyme; Myticin; Mytilin; Defensin; Antimicrobial peptide. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-7345.pdf |
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| Li, H; Parisi, M; Toubiana, Mylene; Cammarata, M; Roch, Philippe. |
| The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 C and cold stress at 5 C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) decrease in the percentage of circulating hemocytes expressing lysozyme mRNA which was now restricted to only small cells, as observed by ISH; and (iv) accumulation of hemocytes expressing lysozyme in the muscle sinus where injection took place. Injection of V.... |
| Tipo: Text |
Palavras-chave: Molluscs; Mytilus; ISH; Q PCR; Gene expression; Lysozyme; Heat shock; Micrococcus lysodeikticus; Vibrio anguillarum; Vibrio splendidus. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4479.pdf |
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| Roch, Philippe; Yang, Y; Toubiana, Mylene; Aumelas, A. |
| Mytilin is a 34-residue antibacterial peptide from the mussel Mytilus galloprovincialis, which in addition possesses in vitro antiviral activity. The three-dimensional solution structure of the synthetic mytilin was established by using 1H NMR and consists of the common cysteine-stabilized alpha beta motif close to the one observed in the mussel defensin MGD-1. Mytilin is characterized by 8 cysteines engaged in four disulfide bonds (2-27, 6-29, 10-31, and 15-34) only involving the beta-strand II. Hydrophilic and hydrophobic areas of mytilin account for 63% and 37%, respectively, a ratio very close to that of MGD-1 (64% and 36%). One linear and three cyclic fragments were designed from the interstrand loop sequence known to retain the biological activities... |
| Tipo: Text |
Palavras-chave: Shrimp; Molluscs; Mussel; Antibacterial activity; WSSV; Viral protection; Beta hairpin structure; NMR; Mytilin; Antimicrobial peptide. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4485.pdf |
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